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HIV Specimen Collection Guide

Test Name Monogram Test N* LabCorp Test N* Clinical Use Viral Load Requirement Specimen Volume
PhenoSense GT* V7000 551690 Determines viral phenotype and genotype resistance to antiretroviral drugs (NRTIs, NNRTI, and Pis). Viral replication capacity is also included. ≥500 copies/mL 3 mL plasma
HIV-1, Quantitative, RT-PCR, with Reflex to PhenoSense GT Graphical: V1000G Nongraphical: V1000N Graphical: 550510 Nongraphical: 550450 Detect and quantitate HIV-1 in plasma with reflex to PhenoSense GT if viral load is ≥500 copies/mL. N/A 6 mL plasma
PhenoSense GT* Plus Integrase* M7000 551920 Determines viral phenotype and genotype resistance to antiviral drugs (NRTIs, NNRTI, Pis, and InIs). ≥500 copies/mL 5 mL plasma
HIV-1, Quantitative, RT-PCR, with Reflex to PhenoSense GT* Plus Integrase* Graphical: V1000G Nongraphical: V1000N Graphical: 550711 Nongraphical: 550625 Detect and quantitate HIV-1 with reflex to PhenoSense GT Plus Integrase if viral load ≥500. NA 6 mL plasma
PhenoSense* V3200 551800 Determines viral phenotype resistance to antiretroviral drugs (NRTIs, NNRTI, and Pis). Viral replication capacity is also included. ≥500 copies/mL 3 mL plasma
HIV-1, Quantitative, RT-PCR, with Reflex to PhenoSense Graphical: V1000G Nongraphical: V1000N Graphical: 550520 Nongraphical: 550470 Detect and quantitate HIV-1 in plasma with reflex to PhenoSense if viral load is ≥500 copies/mL. N/A 6 mL plasma
PhenoSense Integrase* S3200 550230 Determines viral phenotype resistance to integrase inhibitors. Viral replication capacity is also included. ≥500 copies/mL 3 mL plasma
PhenoSense Entry* E2000 550240 Determines viral phenotype resistance to enfuvirtide. ≥1000 copies/mL 3 mL plasma
GenoSure PRime** P5000 551700 Detect resistance in HIV-1 to antiretroviral drugs (NRTIs, NNRTI, Pis, and InIs) ≥500 copies/mL 5 mL plasma
HIV-1, Quantitative, RT-PCR, with Reflex to GenoSure PRime Graphical: V1000G Nongraphical: V1000N Graphical: 550630 Nongraphical: 550655 Detect and quantitate HIV-1 in plasma with reflex to GenoSure PRime if viral load is ≥500 copies/mL. N/A 6 mL plasma
GenoSure® MG* G5000 551697 Detect resistance in HIV-1 to antiretroviral drugs (NRTIs, NNRTI, and Pis) ≥500 copies/mL 5 mL plasma
HIV-1, Quantitative, RT-PCR, with Reflex to GenoSure MG Graphical: V1000G Nongraphical: V1000N Graphical: 550422 Nongraphical: 550432 Detect and quantitate HIV-1 in plasma with reflex to GenoSure MG if viral load is ≥500 copies/mL. N/A 6 mL plasma
GenoSure Archive® R6000 551776 Detect resistance of HIV-1 to protease, reverse transcriptase and integrase inhibitors in cell-associated viral DNA. For patients with an undetectable viral load. 4 mL whole blood
Trofile® DNA E3600 829670 Detect HIV-1 coreceptor tropism to determine eligibility for a CRS antagonist. For patients with an undetectable viral load. 4 mL whole blood
Trofile® E3100 553100 Detect HIV-1 coreceptor tropism to determine eligibility for a CRS antagonist. ≥1000 copies/mL 3 mL plasma
HIV-1, Quantitative, RT-PCR, with Reflex to Trofile® Graphical: V1000G Nongraphical: V1000N Graphical: 550390 Nongraphical: 550310 Detect and quantitate HIV-1 in plasma with reflex to Trofile if viral load is ≥1000 copies/mL. N/A 6 mL plasma
Trofile® Select E3000T 553355 Allows for the determination of HIV-1 coreceptor tropism status, by which the appropriate assay, Trofile® or Trofile® DNA, is selected through an initial prescreening viral assessment. The coreceptor tropism status may be used to determine eligibility for CRS antagonist therapy, such as maraviroc (Selzentry®). N/A 5 mL plasma and 4 mL whole blood
HIV-1, Quantitative, Real-Time PCR (Graphical or Nongraphical) Graphical: V1000G Nongraphical: V1000N Graphical: 550420 Nongraphical: 550430 Quantitative measurement of HIV-1 RNA in plasma. N/A 3.5 mL plasma

*Note: This procedure may not be successful when the HIV viral load is <500 copies/mL plasma. If there is insufficient virus to produce results, HIV-1 RNA quantitation will be performed to confirm viral load, and the procedure will be billed at the account’s prevailing fee.

Plasma Sample Collection and Preparation for Resistance assays and Trofile (excluding Trofile DNA)

  1. Collect whole blood by venipuncture. For tests with sample volumes of 3.0 or 3.5 mLs plasma either:
    • Two EDTA (lavender-top) tubes (preferred)
    • OR Two 5.0 mL PPT (pearl-top) tubes

For tests with sample volumes of 5.0 or 6.0 mLs plasma either:

  1. Spin immediately at 1,000 to 1,200 g for 10 to 15 minutes at room temperature.

  2. Don’t leave samples in centrifuge after spinning.

  3. Prepare for freezing:

    • For PPT samples, freeze directly in tube.
    • Note: Specimens collected in a PPT tube for viral load assays MUST be transferred to a screw-cap tube prior to freezing.
    • OR For EDTA samples, aliquot plasma and transfer to screw-cap tube.

  4. Freeze immediately at -20°C. Sample should be frozen solid before shipping. Note: Do not place samples on dry ice until completely frozen.

Whole Blood Sample Preparation for Trofile® DNA and GenoSure Archive®

  1. Draw 4 mL of whole blood into an EDTA (lavender-top) tube.

  2. DO NOT CENTRIFUGE.

    • Both plasma and whole blood samples must be collected and processed.

CHECKLIST FOR MORE SUCCESSFUL RESULTS

TOP 3 REASONS TEST CANNOT BE PERFORMED:

  1. Low viral load.
  2. Unfrozen specimens or specimens exposed to repeated freeze-thaw cycles.
  3. Unlabeled specimens.